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The translation of certain cellular mRNAs can be initiated by both internal ribosome entry and the capdependent scanning mechanism (Stoneley et al., 2000b; Vagner et al., 1996). It was likely that this would also be the case with the p36 isoform of BAG1 since it has been shown that translational control of the human isoforms of BAG1 involves the scanning mechanism of translation (Packham et al., 1997; Yang et al., 1998). To investigate the contribution the IRES makes to the expression of BAG1 isoforms the DNA encoding the BAG1 5' UTR was inserted into the monocistronic firefly luciferase vector pGL3 (Figure 4a) to create the vector pGBL.

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